Nek2 kinase is highly active during the G2/M phase of the cell cycle. It is involved in several critical processes such as centrosome separation, spindle assembly, and chromosomal alignment. Nek2 phosphorylates various substrates, including centrosome-associated proteins like C-Nap1 and rootletin, which facilitates the separation of centrosomes and ensures proper bipolar spindle formation. This kinase activity is tightly regulated to maintain cellular homeostasis and prevent mitotic errors.
