Studying Nek2 kinase in histology involves multiple approaches. Immunohistochemistry (IHC) is a widely used technique to detect and localize Nek2 in tissue samples. IHC involves the use of specific antibodies that bind to Nek2, followed by visualization using chromogenic or fluorescent labels. Additionally, Western blotting and quantitative PCR can be employed to analyze Nek2 expression levels in tissue extracts. Advanced imaging techniques, such as confocal microscopy, help in studying the spatial distribution of Nek2 within cellular compartments.
