Tissue preparation is a multi-step process that includes fixation, embedding, sectioning, and staining.
1. Fixation: This step involves preserving the tissue to prevent degradation. Common fixatives include formaldehyde and glutaraldehyde, which cross-link proteins and stabilize cellular structures. 2. Embedding: The fixed tissue is embedded in a medium (usually paraffin wax) to provide support during sectioning. 3. Sectioning: Thin slices of the embedded tissue are cut using a microtome, allowing for light to pass through the sample for microscopic examination. 4. Staining: Staining enhances the contrast of the tissue sections, making different structures more distinguishable. Common stains include Hematoxylin and Eosin (H&E), which highlight nuclei and cytoplasmic components, respectively.
