Interpreting line graphs involves understanding the axes, data points, and the overall trend. The x-axis typically represents the independent variable, such as time or treatment conditions, while the y-axis represents the dependent variable, such as cell count or biomarker levels. 1. Slope Analysis: The slope of the line indicates the rate of change. A steeper slope suggests a rapid change, whereas a flatter slope indicates a slower change. 2. Peaks and Troughs: Peaks may indicate maximum values (e.g., highest cell proliferation), while troughs may indicate minimum values (e.g., lowest cell viability). 3. Comparative Lines: Multiple lines on the same graph can represent different groups or conditions, facilitating direct comparison.