What Challenges are Associated with Clear Visualization?

Several challenges can impede clear visualization in histology:

1. Artifact Formation: During tissue preparation, artifacts such as shrinkage, folding, or tearing can occur, potentially misleading the interpretation of tissue morphology.
2. Inadequate Staining: Poor staining techniques can result in weak contrast or non-specific binding, making it difficult to identify specific structures.
3. Resolution Limitations: Even with advanced microscopy, there are limits to the resolution that can be achieved, affecting the ability to visualize very small structures.