Advanced imaging technologies have significantly improved the quality of histological visualization:
1. Light Microscopy: This is the most basic form of microscopy used in histology. It includes brightfield, phase contrast, and fluorescence microscopy. 2. Confocal Microscopy: Confocal microscopy enhances optical resolution and contrast by using point illumination and spatial pinholes to eliminate out-of-focus light. 3. Electron Microscopy: Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) offer high-resolution images of tissue ultrastructure. TEM provides detailed images of thin sections, while SEM gives three-dimensional views of surface structures.
