Several measures can be taken to ensure proper embedding:
Proper Orientation: Careful attention should be given to the orientation of the sample during embedding to ensure accurate representation of the tissue structure. Thorough Processing: Ensuring that each step of the tissue processing protocol, from fixation to dehydration, clearing, and infiltration, is thoroughly performed. Controlled Temperature: Maintaining the paraffin wax at the correct temperature to ensure complete infiltration and to avoid tissue damage. Removing Air Bubbles: Techniques such as vacuum embedding can be used to remove air bubbles, ensuring a homogeneous block. Proper Placement: Ensuring that tissues are placed flat and in the correct orientation within the mold.
