After purification, it is crucial to validate the purity and integrity of the molecules. Common validation techniques include:
1. SDS-PAGE: To check the purity and molecular weight of proteins. 2. Western Blotting: To confirm the presence of the protein of interest. 3. Spectrophotometry: To determine the concentration and purity of nucleic acids. 4. Mass Spectrometry: To analyze the composition and confirm the identity of purified proteins.
