What are the Challenges in Purifying Biological Molecules?
Purifying biological molecules can be challenging due to:
1. Complex Mixtures: Biological samples often contain a vast array of different molecules, making it difficult to isolate the molecule of interest. 2. Degradation: Proteins and nucleic acids can degrade during the purification process, requiring the use of inhibitors and stabilizing agents. 3. Low Abundance: Some molecules are present in very low quantities, necessitating highly sensitive and specific purification methods.