Despite its advantages, live cell imaging presents several challenges:
Phototoxicity: Prolonged exposure to light can damage live cells, necessitating the use of low-intensity light or advanced imaging techniques to minimize harm. Photobleaching: Fluorescent signals can fade over time, reducing image quality. Researchers must use photostable dyes or proteins to mitigate this issue. Environmental Control: Maintaining optimal conditions (e.g., temperature, pH, and humidity) is crucial to ensure the viability of live cells during imaging. Data Management: Live cell imaging generates large amounts of data, requiring robust storage and analysis solutions.
