purification of biological molecules

How is Nucleic Acid Purification Performed?

Purifying nucleic acids involves several steps to ensure high yield and purity:
1. Cell Lysis: Breaking open cells to release nucleic acids.
2. Removal of Proteins: Using proteases or organic solvents to remove proteins contaminating the nucleic acids.
3. Precipitation: Ethanol or isopropanol is commonly used to precipitate nucleic acids.
4. Purification Kits: Commercial purification kits, such as those from Qiagen or Thermo Fisher, streamline the process, offering high purity and yield.

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