How is mTORC1 activation detected in histological samples?
mTORC1 activation can be detected in histological samples using immunohistochemistry (IHC) and immunofluorescence techniques. These methods utilize antibodies that specifically recognize phosphorylated targets of mTORC1, such as phospho-S6K1 or phospho-4E-BP1. Detection of these phosphorylated proteins can indicate active mTORC1 signaling within specific cells or tissues.
