formalin fixation

What are the Disadvantages of Using Formalin?

Despite its advantages, formalin fixation has some drawbacks:
Toxicity: Formaldehyde is a toxic and potentially carcinogenic substance.
Artifact formation: Over-fixation can lead to tissue hardening and shrinkage, creating artifacts.
Antigen masking: Can mask antigens, complicating immunohistochemical analyses.

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