Fixation: Specimens are preserved using chemical fixatives like formalin to prevent decay and maintain tissue structure. Embedding: Fixed tissues are embedded in paraffin wax to create a solid block that can be sliced into thin sections. Sectioning: The paraffin-embedded tissues are cut into thin sections using a microtome, making them suitable for microscopic examination. Staining: Sections are stained with dyes to highlight specific structures or components within the tissue, such as hematoxylin and eosin (H&E) stain. Mounting: Stained sections are mounted on glass slides and covered with a coverslip for observation under a microscope.
