How is Cell Cycle Arrest Detected in Histological Samples?
Histologists use several techniques to detect cell cycle arrest in tissue samples:
Immunohistochemistry (IHC): This technique involves using antibodies to detect specific proteins that are markers of cell cycle arrest, such as p21, p53, and cyclins. Flow cytometry: This method can analyze the DNA content of cells to determine their position in the cell cycle, revealing an accumulation of cells at specific checkpoints. TUNEL assay: This assay detects DNA fragmentation, which can indicate apoptosis following cell cycle arrest.
