How Does Edman Degradation Compare to Other Sequencing Techniques?
Edman degradation remains a classic technique for protein sequencing, particularly for its ability to provide accurate results for the first 5-10 residues of a protein. However, it is relatively slow and labor-intensive compared to modern techniques like mass spectrometry. Mass spectrometry offers rapid, high-throughput analysis and can handle larger proteins and complex mixtures, making it more suitable for large-scale proteomic studies.
