Edman degradation is a method of sequencing amino acids in a peptide. It was developed by Pehr Edman and allows for the sequential identification of amino acids from the N-terminus of a peptide chain. This process involves the use of phenyl isothiocyanate (PITC) which selectively reacts with the N-terminal amino acid to form a cyclic derivative, which can be cleaved without disrupting the peptide bonds of the remaining amino acids.
