chromogenic multiplex staining

What are the Challenges Associated with Chromogenic Multiplex Staining?

- Cross-Reactivity: Non-specific binding and cross-reactivity between antibodies can lead to false positives.
- Signal Interference: Overlapping colors can make it difficult to distinguish between different markers.
- Optimization: Requires careful optimization of antibody concentrations, incubation times, and detection protocols.
- Limited Number of Markers: The number of detectable markers is limited by the availability of distinct chromogens.

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