What are the Challenges Associated with Chromogenic Multiplex Staining?
- Cross-Reactivity: Non-specific binding and cross-reactivity between antibodies can lead to false positives. - Signal Interference: Overlapping colors can make it difficult to distinguish between different markers. - Optimization: Requires careful optimization of antibody concentrations, incubation times, and detection protocols. - Limited Number of Markers: The number of detectable markers is limited by the availability of distinct chromogens.