What are the Limitations of Edman Degradation in Histology?
One significant limitation of Edman degradation is that it is generally effective for small peptides, typically fewer than 50 amino acids. In the complex environment of a tissue sample, proteins are often much larger, requiring prior fragmentation into smaller peptides. Additionally, post-translational modifications and blocked N-termini can hinder the sequencing process. Therefore, it is often complemented with other techniques such as mass spectrometry for comprehensive protein analysis.
