In histology, the primary goal is to prepare tissue samples for microscopic examination. This process often involves several steps, including fixation, dehydration, clearing, embedding, sectioning, and staining. Traditionally, xylene has been used as a clearing agent during the preparation of tissue samples. However, xylene poses significant health risks, including toxicity and carcinogenicity. As a result, there has been a growing interest in finding safer alternatives, with limonene being one of the most promising options.
