What are the Challenges in Library Construction for Histology?
Several challenges can arise during library construction:
- Quality of Tissue Samples: Poorly preserved samples can lead to degraded nucleic acids, affecting the quality of the library. - Biases in Representation: Some sequences may be preferentially amplified, leading to an unbalanced representation in the library. - Technical Variability: Differences in extraction, fragmentation, and amplification protocols can introduce variability, complicating data analysis. - Contamination: Contaminants from reagents or the environment can introduce noise into the dataset, making it harder to discern meaningful biological signals.
