There are several advantages to using limonene in histological procedures:
1. Reduced Health Risks: Unlike xylene, limonene is non-toxic and non-carcinogenic, making it safer for laboratory personnel. 2. Environmental Friendliness: Limonene is biodegradable and derived from renewable resources, which makes it an environmentally sustainable option. 3. Efficiency: Limonene has been shown to perform comparably to xylene in terms of tissue clearing, without compromising the quality of histological sections.