While limonene offers many benefits, there are some potential drawbacks to be aware of:
1. Cost: Limonene can be more expensive than xylene, which might be a consideration for laboratories with limited budgets. 2. Odor: Although generally considered pleasant, the strong citrus odor of limonene can be overpowering in enclosed laboratory spaces. 3. Compatibility: Limonene may not be compatible with all types of mounting media and staining protocols. It is essential to validate its use for specific histological applications.
