resolution decreases with sample size

What Techniques Can Mitigate Resolution Loss?

Several techniques can help mitigate the loss of resolution when working with larger samples:
Sectioning: Careful sectioning of the sample into thinner slices can help maintain higher resolution. This technique is essential for minimizing overlap and light scattering.
Staining: Proper staining techniques can enhance contrast and detail, improving the apparent resolution even in larger samples.
Advanced Imaging Techniques: Utilizing advanced imaging modalities such as confocal microscopy or electron microscopy can significantly improve resolution. These techniques often allow for higher resolution imaging of thicker sections.

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