2d histological techniques

What is the Process of Sample Preparation?

The process of sample preparation for 2D histological techniques typically involves several critical steps:
Fixation: This step preserves the tissue by stabilizing proteins and preventing degradation. Commonly used fixatives include formaldehyde and glutaraldehyde.
Embedding: The fixed tissue is embedded in a medium such as paraffin or resin to provide support for thin sectioning.
Sectioning: The embedded tissue is cut into thin sections using a microtome. Sections are typically 4-10 micrometers thick.
Staining: Stains are applied to enhance contrast and highlight specific structures within the tissue. Hematoxylin and eosin (H&E) is a commonly used staining combination.

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