Why Does Resolution Decrease with Increasing Sample Size?
There are multiple reasons why resolution decreases as the sample size increases:
Optical Limitations: Larger samples may exceed the field of view of the microscope, requiring lower magnification to visualize the entire sample. Lower magnification inherently offers lower resolution. Thickness of Sections: Thicker sections are often used for larger samples, which can lead to overlap of structures and reduced clarity. Thinner sections are essential for high-resolution imaging but are harder to obtain from larger samples. Light Scattering: Larger samples tend to scatter light more, which can blur the image and reduce resolution. This effect is particularly significant in tissues with complex structures.
