How Do Different Microscopes Affect Resolution and Sample Size?
Different types of microscopes offer varying levels of resolution, which can affect the handling of larger samples:
Light Microscopy: Traditional light microscopes have limitations in resolution, especially with larger samples. However, techniques such as fluorescence microscopy can improve detail. Confocal Microscopy: This technique offers higher resolution by using point illumination and spatial filtering. It is particularly useful for thicker sections of larger samples. Electron Microscopy:Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) provide extremely high resolution, making them ideal for detailed study of large samples at the cellular and subcellular levels.
