What Techniques Are Used to Study Gene Function in Tissues?

Several advanced techniques are employed to study gene function in tissues, including:
- Immunohistochemistry: This technique uses antibodies to detect specific proteins in tissue sections, allowing researchers to visualize the expression and localization of gene products.
- In Situ Hybridization: This method uses labeled probes to detect specific mRNA sequences within tissue sections, providing information on gene expression patterns.
- Fluorescence In Situ Hybridization (FISH): FISH is used to detect and localize the presence or absence of specific DNA sequences on chromosomes, aiding in the study of genetic abnormalities.
- RNA Sequencing: Although not a histological technique per se, RNA sequencing of microdissected tissue samples can provide comprehensive profiles of gene expression.