Holocrine secretion is studied using various histological techniques. Hematoxylin and eosin (H&E) staining is widely used to visualize the structure of sebaceous glands and the stages of cell differentiation and disintegration. Electron microscopy provides detailed images of the cellular ultrastructure, including lipid accumulation and membrane rupture. Molecular techniques such as immunohistochemistry can identify specific proteins involved in the apoptotic process and lipid metabolism within sebaceous glands.
