Histology is the study of tissues at the microscopic level, which requires various techniques for sample preparation, staining, and examination. One such concern during these processes is limited penetration. This term refers to the restricted diffusion or infiltration of reagents, stains, or fixatives into tissue samples. Limited penetration can affect the quality of the histological analysis, leading to incomplete or uneven staining, poor fixation, and consequently, inaccurate results.
