How does embedding medium affect tissue sectioning?
The choice of embedding medium is vital for successful tissue sectioning. Paraffin is commonly used due to its ability to provide support to the tissue, allowing thin sections to be cut. The paraffin should be of high quality and maintained at an appropriate temperature (56-58°C) to ensure proper infiltration. Alternatives like resin can be used for specific applications requiring ultra-thin sections, such as electron microscopy. The embedding medium must be compatible with the tissue type and the intended analysis method to ensure optimal sectioning and subsequent examination.
