Several factors can influence the clarity of histological samples: 1. Quality of Fixation: Poor fixation can lead to tissue shrinkage and artifacts, obscuring cellular details. 2. Thickness of Sections: Sections that are too thick may not allow sufficient light to pass through, reducing clarity. 3. Staining Techniques: Inadequate staining can result in poor contrast, making it difficult to differentiate between various tissue components. 4. Mounting Medium: The choice of mounting medium can affect the refractive index and visibility of the tissue sections.
