Histologists may encounter several problems that affect clarity, including: 1. Artifacts: These are distortions or structures not present in the original tissue, caused by processing errors. Common artifacts include air bubbles, folds, and knife marks. 2. Over-Staining or Under-Staining: Both can obscure cellular details, making it challenging to interpret the sample accurately. 3. Inadequate Dehydration: Residual water in the tissue can cause poor clearing and result in opaque sections.
