What Are the Key Steps Involved in Standardized Histology Protocols?
Standardized histology protocols typically involve several critical steps:
Fixation: Preserving tissue morphology and preventing degradation using fixatives like formalin. Processing: Dehydrating and clearing tissues before embedding them in paraffin wax. Embedding: Encasing tissue in a solid medium for sectioning. Sectioning: Cutting thin tissue slices using a microtome. Staining: Applying dyes like hematoxylin and eosin to visualize cellular structures. Mounting: Placing stained sections on slides for microscopic examination.
