Fixation: Preserving the tissue to prevent degradation. Embedding: Encasing the tissue in a solid medium, usually paraffin. Sectioning: Cutting the tissue into thin slices using a microtome. Deparaffinization and Rehydration: Removing paraffin and rehydrating the tissue sections. Staining with Hematoxylin: Immersing the sections in hematoxylin to stain the nuclei. Differentiation: Washing the sections to remove excess stain. Staining with Eosin: Immersing the sections in eosin to stain the cytoplasm and extracellular matrix. Dehydration and Mounting: Dehydrating the stained sections and mounting them on slides for examination.
