In histology, apoptosis inhibition is studied using various techniques, including:
Immunohistochemistry (IHC): This technique is used to detect specific proteins involved in apoptosis and its inhibition within tissue sections. TUNEL Assay: This assay identifies DNA fragmentation, a hallmark of apoptosis, allowing researchers to assess apoptosis levels in tissues. Western blotting: This method analyzes the expression of apoptosis-related proteins in tissue samples. In situ hybridization: This technique detects mRNA levels of apoptosis-related genes in tissue sections. Flow cytometry: Although typically used on cell suspensions, this method can provide insights into apoptosis regulation by analyzing cell populations.