Artifacts can arise during H&E staining, affecting the clarity and accuracy of the tissue examination. Common artifacts include:
Air Bubbles: Often trapped under the coverslip during mounting. Folding: Tissue sections may fold during sectioning or staining, obscuring details. Overstaining or Understaining: Imbalances in staining times can lead to overly intense or weak staining. Fixation Artifacts: Improper fixation can cause tissue shrinkage or distortion.
