What Are the Advantages of CISH Over Other Techniques?
One of the main advantages of CISH is that it allows for the simultaneous visualization of tissue morphology and target nucleic acids. This is particularly useful in histology, where understanding the spatial relationship between the target and the surrounding tissue is crucial. Additionally, CISH does not require specialized fluorescence microscopy equipment, making it more accessible for many laboratories. CISH results are also permanent, unlike fluorescent signals which may fade over time. This permanency allows for long-term storage and re-evaluation of slides. The chromogenic signals produced in CISH are often easier to interpret, especially for pathologists accustomed to traditional staining techniques like Hematoxylin and Eosin (H&E).
