Several key protocols must be followed in histology to ensure compliance:
1. Sample Collection: Proper techniques for tissue biopsy and fixation are essential to preserve the tissue's cellular structure. 2. Tissue Processing: Protocols for dehydration, clearing, and embedding tissues in paraffin or other media must be strictly followed. 3. Sectioning: Using a microtome to cut thin sections of the tissue requires precision to avoid artifacts. 4. Staining: Staining techniques, such as Hematoxylin and Eosin (H&E) staining, must be performed meticulously to differentiate cellular components. 5. Microscopy: The use of light microscopy or other imaging techniques must be standardized to ensure consistent results.
