The deparaffinization process typically involves a series of steps using organic solvents. Here are the main steps:
Xylene Treatment: The tissue sections are first immersed in xylene or a xylene substitute to dissolve the paraffin wax. This step is usually repeated several times to ensure complete removal of the wax. Graded Alcohol Series: After xylene treatment, the sections are passed through a series of decreasing concentrations of alcohol (usually ethanol). This step helps to remove any remaining xylene and prepares the tissue for hydration.
