Hydration involves passing the tissue sections through a series of decreasing concentrations of alcohol, followed by immersion in water. Here are the main steps:
Alcohol Series: The sections are passed through a series of decreasing concentrations of alcohol (ethanol), usually starting from 100% ethanol and moving to 95%, 70%, and finally to water. This step gradually reintroduces water into the tissue. Water Rinse: The final step involves rinsing the tissue sections in distilled or deionized water to remove any remaining alcohol. This step prepares the tissue for staining.
