What are the Common Challenges in Deparaffinization and Hydration?
Several challenges can arise during deparaffinization and hydration, including:
Incomplete Deparaffinization: Insufficient deparaffinization can result in residual wax, leading to poor staining. Ensuring thorough deparaffinization with multiple xylene treatments can help mitigate this issue. Overhydration: Prolonged exposure to water can lead to tissue swelling and damage. Careful monitoring of the hydration steps can prevent overhydration. Alcohol Evaporation: Alcohol can evaporate quickly, especially in open containers, leading to inconsistent concentrations. Using tightly sealed containers and monitoring alcohol levels can help maintain consistency.
