Despite its many advantages, PCR also has some limitations:
Contamination Risk: The high sensitivity of PCR makes it susceptible to contamination, which can lead to false-positive results. Strict laboratory protocols are required to minimize this risk. Quantitative Limitations: While PCR can detect the presence of DNA, accurately quantifying the amount of DNA can be challenging, especially in complex tissue samples. Primer Design: Designing specific and efficient primers for PCR can be difficult, particularly when working with highly variable or unknown DNA sequences.
