pcr (polymerase chain reaction)

What are the Steps Involved in PCR?

PCR typically involves three main steps:
Denaturation: The double-stranded DNA is heated to around 95°C to separate it into two single strands.
Annealing: The temperature is lowered to about 50-65°C to allow short DNA primers to attach to the target DNA sequences.
Extension: The temperature is raised to around 72°C, enabling the Taq polymerase enzyme to synthesize new DNA strands by adding nucleotides to the primers.

Frequently asked queries:

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