What are the Consequences of Improper Dehydration?
Improper dehydration can have several detrimental effects on histological outcomes: - Poor Tissue Morphology: Residual water can cause tissue shrinkage, distortion, and collapse. - Suboptimal Staining: Water left in the tissue can interfere with staining protocols, leading to weak or uneven staining. - Artifact Formation: Incomplete dehydration can lead to the formation of artifacts, such as vacuoles or precipitates, which can obscure histological details. - Poor Sectioning: Water in the tissue can make it difficult to obtain thin, uniform sections, complicating microscopic examination.
