Preventing improper dehydration involves careful attention to the dehydration protocol: - Follow Protocols: Adhere strictly to established protocols regarding ethanol concentrations and immersion times. - Monitor Reagent Quality: Use high-quality, uncontaminated ethanol. Regularly replace reagents to ensure their effectiveness. - Control Tissue Thickness: Ensure tissues are of appropriate thickness for uniform dehydration. - Automated Systems: Consider using automated tissue processors, which can standardize and optimize dehydration steps.
