Several factors can lead to improper dehydration, including: - Incorrect Ethanol Concentrations: Using incorrect ethanol gradients or concentrations can result in incomplete water removal. - Inadequate Time: Insufficient time in each ethanol solution can leave water within the tissue. - Tissue Thickness: Very thick tissue samples may not dehydrate uniformly. - Reagent Quality: Poor quality or contaminated ethanol can hinder effective water removal.
