Despite their advantages, there are some challenges associated with the use of quantum dots in histology:
1. Toxicity: Some quantum dots are made from heavy metals like cadmium, which can be toxic to cells and tissues. This necessitates careful consideration of the type and concentration of quantum dots used. 2. Complex Synthesis and Functionalization: The synthesis of quantum dots and their functionalization with biological molecules (like antibodies) can be complex and require specialized expertise. 3. Cost: Quantum dots can be expensive compared to traditional fluorophores, which may limit their widespread use in routine histological applications.
