Mutation analysis in histology faces several challenges:
1. Sample Quality: Poor-quality samples can lead to inaccurate results. Ensuring high-quality tissue samples is crucial. 2. Complexity of Data: The large amount of data generated, especially by NGS, requires sophisticated bioinformatics tools for analysis. 3. Interpretation: Distinguishing between pathogenic mutations and benign variants can be challenging. 4. Cost: High-throughput techniques like NGS can be expensive, limiting their accessibility.
