What Are the Common Techniques Used in Mutation Analysis?
Several techniques are employed in mutation analysis, including:
1. Polymerase Chain Reaction (PCR): A method to amplify specific DNA sequences. 2. Sanger Sequencing: A traditional method for sequencing DNA. 3. Next-Generation Sequencing (NGS): A high-throughput method that allows for the sequencing of large amounts of DNA quickly and efficiently. 4. Fluorescence In Situ Hybridization (FISH): A technique used to detect and localize specific DNA sequences on chromosomes. 5. Immunohistochemistry (IHC): Used to detect specific proteins in tissue sections, indirectly suggesting mutations.
